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Participants seronegative for HIV-1 had monthly appointments that involved HIV-1 testing, dispensation of thirty days of study medicine, collection of the prior month’s unused medication, individualized adherence counseling, and standardized assessment of sexual behavior and side effects . Serum chemical and hematologic analyses were performed at one month and quarterly thereafter. Women were tested monthly for pregnancy; study medication was withheld in ladies who became pregnant, plus they were referred for antenatal care and allowed to resume study medicine when no more pregnant or lactating. Companions seropositive for HIV-1 were followed quarterly , with HIV-1 principal care solutions administered and with CD4 counts obtained every 6 months.